Michel's Transport Medium as an Alternative to Liquid Nitrogen for PCR Analysis of Skin Biopsy Specimens.

Enhanced diagnostic immunofluorescence using biopsies transported in saline

BACKGROUND The demonstration of tissue-bound immunoreactants by direct immunofluorescence microscopy (DIF) is a valuable parameter in the diagnosis of various autoimmune and immunecomplex-mediated skin diseases. For preservation of tissue-bound immunoreactants, biopsies are usually fresh-frozen in liquid nitrogen or transported in Michel's fixative. But even optimally preserved tissue specimens...

Diagnostic value of direct immunofluorescence of mucosal and skin biopsy specimens on formalin-fixed paraffin-embedded biopsy specimens compared to specimens placed in normal saline as the gold standard method in the diagnosis of a group of autoimmun

Background and Aim: Direct immunofluorescence (DIF) is represented as a gold standard method in diagnosis of autoimmune blistering dermatoses. Normal saline, liquid nitrogen and michel’ solution are a widely accepted media for preserving biopsy samples of skin or mucosa before DIF examination. Occasionally clinicians put the biopsy specimen taken for DIF in formalin 10%, occasionally clinicians...

Identification of Mycobacteria species in cutaneous lesions of Sarcoidosis by PCR-Restriction Ragment Length Polymorphism (PCR-RFLP) method

Background: Sarcoidosis is a granulomatous multisystem disease of unknown etiology. It has recently been tired to detect Mycobacteria genome in biopsy specimens of patients with sarcoidosis by Polymorphism chain reaction method. Objective: To detect and identify Mycobacteria species in cutaneous lesions of the patients with sarcoidosis by PCR-RFLP. Patients and methods: 20 patients with clinica...

Laboratory diagnosis of fungal infections

Selection of appropriate specimens for culture and microscopic examination is based on the results of clinical and radiographic examination and consideration of the most likely fungal pathogen that may cause such an infection. Specimens should be collected under aseptic conditions or after appropriate cleaning and decontamination of the collection site and rapidly transported to the clinical my...

PCR and RFLP Analysis for Identification and Typing of Helicobacter pylori Strains from Gastric Biopsy Specimens